Document Type
Article
Publication Date
2013
Publication Title
Gene Therapy
Volume
20
Issue
2
Pages
151-157
DOI
10.1038/gt.2012.15
Abstract
In vivo gene transfer to the ischemic heart via electroporation holds promise as a potential therapeutic approach for the treatment of heart disease. In the current study, we investigated the use of in vivo electroporation for gene transfer using three different penetrating electrodes and one non-penetrating electrode. The hearts of adult male swine were exposed through a sternotomy. Eight electric pulses synchronized to the rising phase of the R wave of the electrocardiogram were administered at varying pulse widths and field strengths following an injection of either a plasmid encoding luciferase or one encoding green fluorescent protein. Four sites on the anterior wall of the left ventricle were treated. Animals were killed 48 h after injection and electroporation and gene expression was determined. Results were compared with sites in the heart that received plasmid injection but no electric pulses or were not treated. Gene expression was higher in all electroporated sites when compared with injection only sites demonstrating the robustness of this approach. Our results provide evidence that in vivo electroporation can be a safe and effective non-viral method for delivering genes to the heart, in vivo.
Original Publication Citation
Hargrave, B., Downey, H., Strange, R., Murray, L., Cinnamond, C., Lundberg, C., . . . Heller, R. (2013). Electroporation-mediated gene transfer directly to the swine heart. Gene Therapy, 20(2), 151-157. doi:10.1038/gt.2012.15
Repository Citation
Hargrave, Barbara; Downey, Harre; Lundberg, Cathryn; Israel, Annelise; Chen, Yeong-Jer; and Heller, Richard, "Electroporation-Mediated Gene Transfer Directly to the Swine Heart" (2013). Bioelectrics Publications. 181.
https://digitalcommons.odu.edu/bioelectrics_pubs/181
Included in
Biochemistry Commons, Genetics Commons, Medical Sciences Commons
Comments
NOTE: This is the author's post-print version of a work that was published in Gene Therapy. The final version was published as:
Hargrave, B., Downey, H., Strange, R., Murray, L., Cinnamond, C., Lundberg, C., . . . Heller, R. (2013). Electroporation-mediated gene transfer directly to the swine heart. Gene Therapy, 20(2), 151-157. doi:10.1038/gt.2012.15
Available at: http://dx.doi.org/10.1038/gt.2012.15