Document Type
Article
Publication Date
2011
Publication Title
Apoptosis
Volume
16
Issue
4
Pages
382-393
DOI
10.1007/s10495-010-0572-y
Abstract
Stimuli directed towards activation of apoptosis mechanisms are an attractive approach to eliminate evasion of apoptosis, a ubiquitous cancer hallmark. In these in vitro studies, kinetics and electric field thresholds for several apoptosis characteristics are defined in E4 squamous carcinoma cells (SCC) exposed to ten 300 ns pulses with increasing electric fields. Cell death was [95% at the highest electric field and coincident with phosphatidylserine externalization, caspase and calpain activation in the presence and absence of cytochrome c release, decreases in Bid and mitochondria membrane potential (Δψm) without apparent changes reactive oxygen species levels or in Bcl2 and Bclxl levels. Bid cleavage was caspase-dependent (55–60%) and calcium-dependent (40–45%). Intracellular calcium as an intrinsic mechanism and extracellular calcium as an extrinsic mechanism were responsible for about 30 and 70% of calcium dependence for Bid cleavage, respectively. The results reveal electric field-mediated cell death induction and progression, activating pro-apoptoticlike mechanisms and affecting plasma membrane and intracellular functions, primarily through extrinsic-like pathways with smaller contributions from intrinsic-like pathways. Nanosecond second pulsed electric fields trigger heterogeneous cell death mechanisms in E4 SCC populations to delete them, with caspase-associated cell death as a predominant, but not an unaccompanied event.
Original Publication Citation
Ren, W., & Beebe, S. J. (2011). An apoptosis targeted stimulus with nanosecond pulsed electric fields (nsPEFs) in E4 squamous cell carcinoma. Apoptosis, 16(4), 382-393. doi:10.1007/s10495-010-0572-y
Repository Citation
Ren, Wei and Beebe, Stephen J., "An Apoptosis Targeted Stimulus with Nanosecond Pulsed Electric Fields (nsPEFs) in E4 squamous Cell Carcinoma" (2011). Bioelectrics Publications. 186.
https://digitalcommons.odu.edu/bioelectrics_pubs/186
ORCID
0000-0002-6075-9452 (Beebe)
Included in
Biochemistry Commons, Biomedical Commons, Cell Biology Commons, Molecular Biology Commons
Comments
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