Document Type

Article

Publication Date

2012

Publication Title

ISRN Dentistry

Volume

2012

Pages

Artricle ID 295736

DOI

10.5402/2012/295736

Abstract

The hypothesis that a cold plasma jet has the antimicrobial effect against Enterococcus faecalis biofilms was tested in vitro. 27 hydroxyapatite discs were incubated with E. faecalis for six days to form a monoculture biofilm on the disc surface. The prepared substrata were divided into three groups: the negative control, the positive control (5.25% NaOCl solution), and the plasma treatment group. Resultant colony-forming unit counts were associated with observations of bacterial cell morphology changes using scanning electron microscopy (SEM). Treatment of E. faecalis biofilm with the plasma and 5.25% NaOCl for 5min resulted in 93.1% and 90.0% kill (P < 0.0001), respectively. SEM detected that nearly no intact bacteria were discernible for the plasmaexposed HA disc surfaces. The demonstrated bactericidal effect of the plasma with direct surface contact may be due to the enhanced oxidation by the locally produced reactive plasma species.

Original Publication Citation

Jiang, C., Schaudinn, C., Jaramillo, D.E., Webster, P., & Costerton, J.W. (2012). In vitro antimicrobial effect of a cold plasma jet against enterococcus faecalis biofilms. ISRN Dentistry, 2012.

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