Diffuse, Non-Polar Electropermeabilization and Reduced Propidium Uptake Distinguish the Effect of Nanosecond Electric Pulses

Authors

Iurii Semenov, Old Dominion UniversityFollow
Christian W. Zemlin, Old Dominion UniversityFollow
Olga N. Pakhomova, Old Dominion UniversityFollow
Shu Xiao, Old Dominion UniversityFollow
Andrei G. Pakhomov, Old Dominion UniversityFollow

Document Type

Article

Publication Date

2015

Publication Title

Biochimica Et Biophysica Acta

Volume

1848

Issue

10

Pages

2118-2125

DOI

10.1016/j.bbamem.2015.06.018

Abstract

Ca²⁺ activation and membrane electroporation by 10-ns and 4-ms electric pulses (nsEP and msEP) were compared in rat embryonic cardiomyocytes. The lowest electric field which triggered Ca²⁺ transients was expectedly higher for nsEP (36 kV/cm)than forms EP (0.09 kV/cm) but the respective doses were similar (190 and460 mJ/g). At higher intensities, both stimuli triggered prolonged firing in quiescent cells. An increase of basal Ca²⁺ level by N10 nM in cells with blocked voltage-gated Ca²⁺ channels and depleted Ca²⁺ depot occurred at 63 kV/cm (nsEP) or 0.14 kV/cm (msEP) and was regarded as electroporation threshold. These electric field values were at 150–230% of stimulation thresholds for both msEP and nsEP, notwithstanding a 400,000-fold difference in pulse duration. For comparable levels of electroporative Ca²⁺ uptake, msEP caused at least 10-fold greater uptake of propidium than nsEP, suggesting increased yield of larger pores. Electroporation by msEP started Ca²⁺ entry abruptly and locally at the electrode-facing poles of cell, followed by a slow diffusion to the center. In a stark contrast, nsEP evoked a “supra-electroporation” pattern of slower but spatially uniform Ca²⁺ entry. Thus nsEP and msEP had comparable dose efficiency, but differed profoundly in the size and localization of electropores.

Comments

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Original Publication Citation

Semenov, I., Zemlin, C., Pakhomova, O. N., Xiao, S., & Pakhomov, A. G. (2015). Diffuse, non-polar electropermeabilization and reduced propidium uptake distinguish the effect of nanosecond electric pulses. Biochimica Et Biophysica Acta, 1848(10), 2118-2125. doi:10.1016/j.bbamem.2015.06.018

Repository Citation

Semenov, Iurii; Zemlin, Christian W.; Pakhomova, Olga N.; Xiao, Shu; and Pakhomov, Andrei G., "Diffuse, Non-Polar Electropermeabilization and Reduced Propidium Uptake Distinguish the Effect of Nanosecond Electric Pulses" (2015). Bioelectrics Publications. 202.
https://digitalcommons.odu.edu/bioelectrics_pubs/202

ORCID

0000-0002-0302-1355 (Semenov), 0000-0003-4950-4130 (Pakhomova), 0000-0002-0375-8373 (Xiao)