Document Type

Article

Publication Date

2005

Publication Title

Biophysical Journal

Volume

89

Issue

1

Pages

274-284

DOI

10.1529/biophysj.104.054494

Abstract

Electric pulses across intact vesicles and cells can lead to transient increase in permeability of their membranes. We studied the integrity of these membranes in response to external electric pulses of high amplitude and submicrosecond duration with a primary aim of achieving selective permeabilization. These effects were examined in two separate model systems comprising of 1), a mixed population of 1,2-di-oleoyl-sn-glycero-3-phosphocholine phospholipid vesicles and in 2), single COS-7 cells, in which large endosomal membrane vacuoles were induced by stimulated endocytosis. It has been shown that large and rapidly varying external electric fields, with pulses shorter than the charging time of the outer-cell membrane, could substantially increase intracellular fields to achieve selective manipulations of intracellular organelles. The underlying principle of this earlier work is further developed and applied to the systems studied here. Under appropriate conditions, we show preferential permeabilization of one vesicle population in a mixed preparation of vesicles of similar size distribution. It is further shown that large endocytosed vacuoles in COS-7 cells can be selectively permeabilized with little effect on the integrity of outer cell membrane.

Comments

Web of Science: "Free full-text from publisher." Elsevier open archive.

(c) 2005 by the Biophysical Society

Original Publication Citation

Tekle, E., Oubrahim, H., Dzekunov, S. M., Kolb, J. F., Schoenbach, K. H., & Chock, P. B. (2005). Selective field effects on intracellular vacuoles and vesicle membranes with nanosecond electric pulses. Biophysical Journal, 89(1), 274-284. doi:10.1529/biophysj.104.054494

ORCID

0000-0002-0434-5001 (Kolb)

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