Document Type
Article
Publication Date
8-2012
Publication Title
PloS One
Volume
7
Issue
8
DOI
10.1371/journal.pone.0043213
Abstract
Treatment of cancer often involves uses of multiple therapeutic strategies with different mechanisms of action. In this study we investigated combinations of nanosecond pulsed electric fields (nsPEF) with low concentrations of gemcitabine on human oral cancer cells. Cells (Cal-27) were treated with pulse parameters (20 pulses, 100 ns in duration, intensities of 10, 30 and 60 kV/cm) and then cultured in medium with 0.01 mu g/ml gemcitabine. Proliferation, apoptosis/necrosis, invasion and morphology of those cells were examined using MTT, flow cytometry, clonogenics, transwell migration and TEM assay. Results show that combination treatments of gemcitabine and nsPEFs exhibited significant synergistic activities versus individual treatments for inhibiting oral cancer cell proliferation and inducing apoptosis and necrosis. However, there was no apparent synergism for cell invasion. By this we demonstrated synergistic inhibition of Cal-27 cells in vitro by nsPEFs and gemcitabine. Synergistic behavior indicates that these two treatments have different sites of action and combination treatment allows reduced doses of gemcitabine and lower nsPEF conditions, which may provide better treatment for patients than either treatment alone while reducing systemic toxicities.
Original Publication Citation
Wang, J., Guo, J.S., Wu, S., Feng, H.Q., Sun, S.J., Pan, J., . . . Beebe, S.J. (2012). Synergistic effects of nanosecond pulsed electric fields combined with low concentration of gemcitabine on human oral squamous cell carcinoma in vitro. PLoS One, 7(8). doi: 10.1371/journal.pone.0043213
Repository Citation
Wang, Jing; Guo, Jinsong; Wu, Shan; Feng, Hongqing; Sun, Shujun; Pan, Jie; Zhang, Jue; and Beebe, Stephen J., "Synergistic Effects of Nanosecond Pulsed Electric Fields Combined with Low Concentration of Gemcitabine on Human Oral Squamous Cell Carcinoma In Vitro" (2012). Bioelectrics Publications. 24.
https://digitalcommons.odu.edu/bioelectrics_pubs/24