Document Type

Article

Publication Date

2019

Publication Title

Cancer Cell International

Volume

19

Issue

135

Pages

1-12

DOI

10.1186/s12935-019-0856-4

Abstract

Background

Acute myeloid leukemia (AML) is a typically fatal malignancy and new drug and treatment need to be developed for a better survival outcome. Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in various of cancer treatment. However, the changes in cell metabolism after CAP treatment of leukemia cells have been rarely studied.

Methods

In this study, we investigated the metabolite profiling of plasma treatment on leukemia cells based on Gas Chromatography Tandem Time-of-Flight Mass Spectrometry (GC-TOFMS). Simultaneously, we conducted a series of bioinformatics analysis of metabolites and metabolic pathways with significant differences after basic data analysis.

Results

800 signals were detected by GC-TOF mass-spectrometry and then evaluated using PCA and OPLS-DA. All the differential metabolites were listed and the related metabolic pathways were analyzed by KEGG pathway. The results showed that alanine, aspartate and glutamate metabolism had a significant change after plasma treatment. Meanwhile, d-glutamine and d-glutamate metabolism were significantly changed by CAP. Glutaminase activity was decreased after plasma treatment, which might lead to glutamine accumulation and leukemia cells death.

Conclusions

We found the above two metabolic pathways vulnerable to plasma treatment, which might result in leukemia cells death and might be the cornerstone of further exploration of plasma treatment targets.

Comments

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and source, provide a link to the creative commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver applies to the data made available in this article, unless otherwise stated.

Original Publication Citation

Xu, D., Ning, N., Xu, Y., Wang, B., Cui, Q., Liu, Z., . . . Kong, M. G. (2019). Effect of cold atmospheric plasma treatment on the metabolites of human leukemia cells. Cancer Cell International, 19(135), 1-12. doi:10.1186/s12935-019-0856-4

12935_2019_856_MOESM1_ESM (1).xlsx (61 kB)
Metabolite mapping

12935_2019_856_MOESM2_ESM.xlsx (45 kB)
Differential metabolites

12935_2019_856_MOESM3_ESM.xlsx (15 kB)
KEGG pathway

12935_2019_856_MOESM4_ESM.xlsx (33 kB)
Pathway analysis

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