Document Type
Article
Publication Date
2021
Publication Title
Cancers
Volume
13
Issue
5
Pages
1-14
DOI
10.3390/cancers13051042
Abstract
Muscle-invasive bladder cancer (MIBC) is a fast-growing and aggressive malignant tumor in urinary system. Since chemotherapy and immunotherapy are only useable with a few MIBC patients, the clinical treatment of MIBC still faces challenges. Here, we examined the feasibility of plasma-activated saline (PAS) as a fledgling therapeutic strategy for MIBC treatment. Our data showed that plasma irradiation could generate a variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in saline. In vivo tests revealed that pericarcinomatous tissue injection with PAS was effective at preventing subcutaneous bladder tumor growth, with no side effects to the visceral organs after long-term administration, as well as having no obvious influence on the various biochemistry indices of the blood in mice. The in vitro studies indicated that adding 30% PAS in cell culture media causes oxidative damage to the bladder transitional cells T24 and J82 through enhancing the intracellular ROS level, and eventually induces cancer cells' apoptosis by activating the ROS-mediated Fas/CD95 pathway. Therefore, for an intracavity tumor, these initial observations suggest that the soaking of the tumor tissue with PAS by intravesical perfusion may be a novel treatment option for bladder cancer.
Original Publication Citation
Zhang, H., Zhang, J. S., Guo, B., Chen, H. L., Xu, D. H., & Kong, M. G. (2021). The antitumor effects of plasma-activated saline on muscle-invasive bladder cancer cells in vitro and in vivo demonstrate Its feasibility as a potential therapeutic approach. Cancers, 13(5), 1-14, Article 1042. https://doi.org/10.3390/cancers13051042
Repository Citation
Zhang, Hao; Zhang, Jishen; Guo, Bo; Chen, Hailan; Xu, Dehui; and Kong, Michael G., "The Antitumor Effects of Plasma-Activated Saline on Muscle-Invasive Bladder Cancer Cells in Vitro and in Vivo Demonstrate Its Feasibility as a Potential Therapeutic Approach" (2021). Bioelectrics Publications. 303.
https://digitalcommons.odu.edu/bioelectrics_pubs/303
ORCID
0000-0001-8142-4726 (Kong)
Comments
© 2021 The Authors
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) license.