Document Type
Article
Publication Date
2021
Publication Title
PLOS One
Volume
16
Issue
9
Pages
e0257287 (1-13)
DOI
10.1371/journal.pone.0257287
Abstract
Aims
Recently, a new defibrillation modality using nanosecond pulses was shown to be effective at much lower energies than conventional 10 millisecond monophasic shocks in ex vivo experiments. Here we compare the safety factors of 300 nanosecond and 10 millisecond shocks to assess the safety of nanosecond defibrillation.
Methods and results
The safety factor, i.e. the ratio of median effective doses (ED50) for electroporative damage and defibrillation, was assessed for nanosecond and conventional (millisecond) defibrillation shocks in Langendorff-perfused New Zealand white rabbit hearts. In order to allow for multiple shock applications in a single heart, a pair of needle electrodes was used to apply shocks of varying voltage. Propidium iodide (PI) staining at the surface of the heart showed that nanosecond shocks had a slightly lower safety factor (6.50) than millisecond shocks (8.69), p = 0.02; while PI staining cross-sections in the electrode plane showed no significant difference (5.38 for 300 ns shocks and 6.29 for 10 ms shocks, p = 0.22).
Conclusions
In Langendorff-perfused rabbit hearts, nanosecond defibrillation has a similar safety factor as millisecond defibrillation, between 5 and 9, suggesting that nanosecond defibrillation can be performed safely.
Original Publication Citation
Neuber, J. U., Pakhomov, A. G., & Zemlin, C. W. (2021). Electroporation safety factor of 300 nanosecond and 10 millisecond defibrillation in Langendorff-perfused rabbit hearts. PLOS One, 16(9), 1-13, Article e0257287. https://doi.org/10.1371/journal.pone.0257287
Repository Citation
Neuber, Johanna U.; Pakhomov, Andrei G.; and Zemlin, Christian W., "Electroporation Safety Factor of 300 Nanosecond and 10 Millisecond Defibrillation in Langendorff-Perfused Rabbit Hearts" (2021). Bioelectrics Publications. 315.
https://digitalcommons.odu.edu/bioelectrics_pubs/315
ORCID
0000-0001-5834-5544 (Zemlin)
Comments
© 2021 Neuber et al.
This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.