Document Type

Article

Publication Date

2021

Publication Title

Frontiers in Oncology

Volume

11

Pages

552480 (1-10)

DOI

10.3389/fonc.2021.552480

Abstract

Cold atmospheric plasma, including plasma jet and surface plasma, can promote the apoptosis of cancer cells without causing significant damage to surrounding normal cells, which was hopeful to be applied to the clinical cancer therapy. However, experimental plasma devices used directly to clinical experiments has challenges in technology and methods, especially the difference in killing tumor cells efficiency of these two common plasma sources. Therefore, it is great necessity to explore the differences in treating tumors between different plasma sources. This paper achieved good killing efficiency by using two kinds of cold atmospheric plasma generating devices, namely plasma jet and surface plasma treatment along acute myeloid leukemia (AML). The results showed that the He plasma jet kills leukemia cells more efficiently than surface plasma with the same voltage and frequency and the same time. By GC-TOFMS and metabolomics analysis, this paper compared the differential metabolites of leukemia cells treated by two plasma devices and the key metabolic pathways closely related to differential metabolites. Simultaneously, we found alanine, aspartate and glutamate metabolism was most correlated with a key differential metabolite, glutamine. It was found that the glutaminase activity of He plasma jet group was lower than that of surface plasma group, which might be a reason for He plasma jet group to kill tumor cells better. It was also worth noting that relative quantity of glucose metabolites of plasma jet treatment group was lower than that of surface plasma treatment group. This study provides the basis for clinical trials for future.

Comments

© 2021 Xu, Ning, Xu, Xia, Liu, Chen and Kong.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Original Publication Citation

Xu, D., Ning, N., Xu, Y., Xia, W., Liu, D., Chen, H., & Kong, M. G. (2021). Effect of He plasma jet versus surface plasma on the metabolites of acute myeloid leukemia cells. Frontiers in Oncology, 11, 1-10, Article 552480. https://doi.org/10.3389/fonc.2021.552480

ORCID

0000-0002-5766-0531 (Chen), 0000-0001-8142-4726 (Kong)

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