Document Type
Presentation
Publication Date
2016
Pages
1-67
DOI
10.13140/RG.2.2.14313.90726
Abstract
Exposure to nanosecond electric pulses (nsEPs) evokes transient rise of cytosolic free Ca²⁺ concentration ([Ca²⁺]i) in the cells. We quantified the external Ca²⁺ uptake, intracellular Ca²⁺ release and uncover interplay of these processes for CHO-K1 cells upon exposure to single 60 ns pulse in the range of amplitudes from 0 to 30 kV/cm. NsEPs are distinguished by the ability to electroporate intracellular membranes. We studied how duration of nsEPs is related to efficiency of intracellular membranes electroporation comparing ability of single 300-, 60- and 10 ns pulses to recruit Ca²⁺ from endoplasmic reticulum (ER) in respect to the uptake of extracellular Ca²⁺ used as a control. Finally, we compared dynamic of Ca²⁺ uptake upon exposure to single conventional (4 ms) EP with nsEP (10 ns) to test hypothesis of supra-electroporation implying different mechanism of pores formation by nsEP.
Rights
© 2016 The Authors. All rights reserved.
Included with the kind written permission of the author.
Original Publication Citation
Semenov, I. (2016). Analysis of Ca²⁺ mobilization of nanosecond pulses in cells. Old Dominion University. https://doi.org/10.13140/RG.2.2.14313.90726
Repository Citation
Semenov, Iurii, "Analysis of Ca²⁺ Mobilization of Nanosecond Pulses in Cells" (2016). Bioelectrics Publications. 386.
https://digitalcommons.odu.edu/bioelectrics_pubs/386
ORCID
0000-0002-0302-1355 (Semenov)