Document Type

Article

Publication Date

2012

Publication Title

Journal of Membrane Biology

Volume

245

Issue

10

Pages

611-616

DOI

10.1007/s00232-012-9472-0

Abstract

We investigated the effects of nanosecond pulse electric fields (nsPEFs) on Jurkat and PANC1 cells, which are human carcinoma cell lines, in the presence of Tween 80 (T80) at a concentration of 0.18% and demonstarted an enhanced killing effect. We used two biological assays to determine cell viability after exposing cells to nsPEFs in the presence of T80 and observed a significant increase in the killing effect of nsPEFs. We did not see a toxic effect of T80 when cells were exposed to surfactant alone. However, we saw a synergistic effect when cells exposed to T80 were combined with the nsPEFs. Increasing the time of exposure for up to 8 h in T80 led to a significant decrease in cell viability when nsPEFs were applied to cells compared to control cells. We also observed cell type-specific swelling in the presence of T80. We suggest that T80 acts as an adjuvant in facilitating the effects of nsPEFs on the cell membrane; however, the limitations of the viability assays were addressed. We conclude that T80 may increase the fragility of the cell membrane, which makes it more susceptible to nsPEF-mediated killing.

Comments

NOTE: This is the final author’s version (post-print) of a work that was published in Journal of Membrane Biology. The final version was published as:

Basu, G., Kalluri, B.S., Sabuncu, A.C., Osgood, C.J., & Stacey, M.W. (2012). Enhanced killing effect of nanosecond pulse electric fields on PANC1 and Jurkat cell lines in the presence of Tween 80. Journal of Membrane Biology, 245(10), 611-616. doi: 10.1007/s00232-012-9472-0

The final publication is available at Springer via http://dx.doi.org/10.1007/s00232-012-9472-0

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