Document Type

Article

Publication Date

2011

Publication Title

Bioelectrochemistry

Volume

82

Issue

2

Pages

131-34

DOI

10.1016/j.bioelechem.2011.06.002

Abstract

We investigated the effects of nanosecond pulsed electric fields (nsPEF) on three human cell lines and demonstrated cell shrinkage, breakdown of the cytoskeleton, nuclear membrane and chromosomal telomere damage. There was a differential response between cell types coinciding with cell survival. Jurkat cells showed cytoskeleton, nuclear membrane and telomere damage that severely impacted cell survival compared to two adherent cell lines. Interestingly, disruption of the actin cytoskeleton in adherent cells prior to nsPEF exposure significantly reduced cell survival. We conclude that nsPEF applications are able to induce damage to the cytoskeleton and nuclear membrane. Telomere sequences, regions that tether and stabilize DNA to the nuclear membrane, are severely compromised as measured by a pan-telomere probe. Internal pore formation following nsPEF applications has been described as a factor in induced cell death. Here we suggest that nsPEF induced physical changes to the cell in addition to pore formation need to be considered as an alternative method of cell death. We suggest nsPEF electrochemical induced depolymerization of actin filaments may account for cytoskeleton and nuclear membrane anomalies leading to sensitization.

Comments

NOTE: This is the final author’s version (post-print) of a work that was published in Bioelectrochemistry. The final version was published as:

Stacey, M., Fox, P., Buescher, S., & Kolb, J. (2011). Nanosecond pulsed electric field induced cytoskeleton, nuclear membrane and telomere damage adversely impact cell survival. Bioelectrochemistry, 82(2), 131-134. doi: 10.1016/j.bioelechem.2011.06.002

The final publication is available at: 10.1016/j.bioelechem.2011.06.002

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