Date of Award
1977
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
Program/Concentration
Biology
Committee Director
James C. Johnson
Committee Member
Lloyd Wolfinbarger, Jr.
Committee Member
Thomas Sitz
Call Number for Print
Special Collections LD4331.B46 A77
Abstract
Duck plague is a hemorrhagic, necrotic disease found exclusively in the order Anseriformes, family Anatidae (ducks, geese, swans). The etiologic agent has been reported to be a herpes-like virus. The Holland strain of duck plague was found to induce cytopathic effects in cell culture (perinuclear vacuolation followed by lysis). A simple reproducible plaque assay for quantitating the virus was developed. Under conditions of the plaque assay, one-hit kinetics were observed. The host range of duck plague virus in cell culture was found to include the order Galliformes, family Phasianidae (pheasants, quails and partridges). Duck plague virus-induced cell culture cytopathic effects were inhibited by phosphonoacetic acid. High concentrations of phosphonoacetic acid did not visably affect duck embryo fibroblast morphology or multiplication. Opaque plaques resulted following the delayed addition of phosphonoacetic acid to infected cultures. It is speculated that opaque plaques were the result of an aborted viral infection.
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DOI
10.25777/aqk8-px88
Recommended Citation
Attanasio, Ronald.
"Duck Plague Virus an Investigation of Cytopathic Effects, in-vitro Host Range, and Inhibition by Phosphonoacetic Acid"
(1977). Master of Science (MS), Thesis, Biological Sciences, Old Dominion University, DOI: 10.25777/aqk8-px88
https://digitalcommons.odu.edu/biology_etds/136
Included in
Animal Diseases Commons, Ornithology Commons, Zoology Commons