Date of Award

Fall 1979

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Carl W. Erkenbrecher, Jr.

Committee Member

Lloyd Wolfinbarger, Jr.

Committee Member

Paul W. Kirk, Jr.

Call Number for Print

Special Collections LD4331.B46 F63

Abstract

Methodologies for the improved recovery of selected pathogenic bacteria from estuarine sediments were investigated. Laboratory and field studies were conducted to document the sensitivity of the techniques for isolating Salmonella species, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Vibrio parahaemolyticus-like organisms were enriched in a seawater yeast extract broth and isolated from Thiosulfate Citrate Bile Salts Sucrose plated media. Enrichment procedures with and without elevated temperatures and subsequent transfers to selective and differential plated media have been examined for the isolation of Salmonella species and P. aeruginosa. These pathogens were initially assayed for utilizing primary enrichments of asparagine broth and dulcitol selenite enrichment broth (DSE). Because of repeated failures to isolate Salmonella species with these latter techniques, additional laboratory studies involving tetrathionate broth, dulcitol phenol red broth, and DSE broth with reduced concentrations of sodium selenite were investigated. Although these modified techniques appeared to be adequate, the DSE selective enrichment procedure proved to be superior for the isolation and enumeration of P. aeruginosa and Salmonella species. Increased sediment inocula in DSE enrichments demonstrated an increased recovery and permitted quantitative enumeration of Salmonella species in these estuarine sediments.

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DOI

10.25776/y0xw-4804

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