Effect of Cocaine on Rabbit Renin Angiotensin System: Cocaine Detection in Adult and Fetal Tissue and Adult Plasma Using Ion Mobility Spectrometry

Author

Barbara Belinda Guinn, Old Dominion University

Date of Award

Summer 1997

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Barbara Hargrave

Committee Member

Wayne Hynes

Committee Member

Christopher Osgood

Committee Member

Edward Poziomek

Committee Member

Lloyd Wolfinbarger

Call Number for Print

Special Collections LD4331.B47 G85

Abstract

Ion mobility spectrometry (IMS) was used to detect cocaine and/or the metabolite ecgonine methyl ester (EME) in tissue and plasma samples from male, maternal, and fetal New Zealand White (NZW) rabbits. EME was consistently detected in all adult plasma samples 5 minutes after the injection of cocaine hydrochloride (cocaine•HCI, 2 mg/kg). EME was also in the liver, kidneys, testicles, and ovaries. Five minutes after the injection of cocaine•HCI, the male arterial pressure (MAP) increased from a mean control value of 79 ± 3.2 mmHg to 88 ± 4.1 mmHg, and the pCO₂ increased from a control value of28 ± 2.4 mmHg to 32 ± 1.7 mmHg. Maternal arterial pressure (MAP) increased from a mean control value of71 ± 5.6 mmHg to 90 ± 3.1 mmHg. Maternal pH and pCO₂ decreased from a control value of 7.44 ± 0.01 to 7.34 ± 0.02 and 98 ± 3.6 mmHg to 83 ± 6.4 mmHg, respectively. Maternal pCO₂ increased from 28 ± 2 mmHg to 32 ± 2 mmHg. The change in plasma renin activity in the male rabbits was not statistically significant. However, female plasma renin activity increased from a control value of 2.7 ± 1.0 ng/mL/h to 9.5 ± 1.1 ng/mL/h. IMS was found to be a useful analytical tool for the detection and identification of EME in adult plasma and in male, female, and fetal heart, lungs, liver, and kidneys. Cocaine, at a dose of 2 mg/kg, alters the activity of the renin angiotensin aldosterone system (RAAS) in maternal rabbits.

Rights

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DOI

10.25777/h174-6z19

Recommended Citation

Guinn, Barbara B.. "Effect of Cocaine on Rabbit Renin Angiotensin System: Cocaine Detection in Adult and Fetal Tissue and Adult Plasma Using Ion Mobility Spectrometry" (1997). Master of Science (MS), Thesis, Biological Sciences, Old Dominion University, DOI: 10.25777/h174-6z19
https://digitalcommons.odu.edu/biology_etds/198