Date of Award

Spring 2000

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Lioyd Wolfinbarger, Jr.

Committee Member

Robert Ratzlaff

Committee Member

Christopher Osgood

Call Number for Print

Special Collections LD4331.B46 K33

Abstract

The microbial etiology of periodontal diseases has led to widespread research in the development of methods and local delivery systems to increase the efficacy of antibiotic therapy. Several drug delivery systems employing biodegradable and nonbiodegradable carriers have been shown to release antibiotics directly into periodontal pockets. The purpose of this study was to determine the binding and release kinetics of tetracycline by demineralized bone. Further aspects of the study include in vitro evaluation of DFDBA (demineralized freeze-dried bone allografts) as a tetracycline carrier system for periodontal therapy. Experiments were performed which employed different tetracycline concentrations with constant amounts of bone and constant tetracycline concentrations with different amounts of bone to determine the kinetics of tetracycline binding. Time course studies of tetracycline binding and release by bone were performed to assess binding capacity and release as a function of time. DFDBA was demonstrated to maximally bind 0.014 mg tetracycline per mg dry weight of DFDBA. Thirty percent of the tetracycline bound to DFDBA was released in a time dependent manner over a four hour incubation period. The rate of tetracycline release was curvilinear over the incubation period studied. The rate of release exhibited a rapid linear decrease for the first 40 minutes and followed a very slow and nonlinear decrease over the remaining 200 minutes suggesting DFDBA may act as a slow release device for the tetracycline.

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In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

DOI

10.25777/d798-xa15

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