Date of Award

Summer 1988

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Keith A. Carson

Committee Member

R. James Swanson

Committee Member

Robert Ratzlaff

Call Number for Print

Special Collections LD4331.B46 W66

Abstract

A cytoarchitectural analysis of the rat olfactory tubercle using Nissl-stained coronal sections revealed that the dense cell layer (DCL) consisted of medium sized striatal cells in the cortical regions and small sized "granule" cells in the cap regions. Also delineated from this experiment was a rim of neuropil, nearly devoid of neurons as well as neuronal processes, outlining the islands of Calleja. Acetylcholinesterase (AChE) localization utilizing light microscopy revealed that the olfactory tubercles (OT) contained AChE-positive fibers that were orientated dorsoventrally in the molecular and multiform layers. The DCL consisted of only fibers en passant and putative terminals. Diisopropylfluorophosphate (DFP) pharmacohistochemical analysis of the rat OT demonstrated that the majority of neurons in the rat OT were medium sized and oval. Also they were found in the multiform layer and not associated with the islands of Calleja. AChE localization using electron microscopy revealed that the reaction product in the molecular layer of the OT was associated with unmyelinated axons, neuronal processes, and symmetrical and asymmetrical contacts, as well as glial processes. The AChE reaction product in the DCL was associated with the membranes of unmyelinated axons as well as the membranes of AChE-negative neurons that constituted this layer. Granule cells that contained a less intense localization of AChE reaction product in the cytoplasm were also observed in this area. The multiform layer consisted of AChE-positive neurons that contained AChE reaction product within the golgi, rough endoplasmic reticulum, and nuclear membrane. AChE was also seen associated with the axolemma of unmyelinated axons, myelinated axons, the membranes of neuronal processes and within synaptic clefts of symmetrical and asymmetrical contacts. Lastly, the reaction product was observed associated with the cytoplasm of capillary endothelial cells.

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DOI

10.25777/r7zh-j121

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