Date of Award

1986

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Lloyd Wolfinbarger, Jr.

Committee Member

Andrew Gordon

Committee Member

Paul Homsher

Call Number for Print

Special Collections LD4331.B46 B74

Abstract

An improved and efficient electrophoretic procedure for the isolation of mitochondrial DNA (mtDNA) is described. The solid phase extraction procedure for the isolation of mtDNA involves embedding as few as 200,000 Ehrlich ascites tumor cells into a block of agarose, digestion of cellular membranes by detergent action and subsequent electrophoretic separation of nucleic acids in agarose gels. Genomic DNA remains in the original block of agarose while the mtDNA migrates in the separation gel to a position equivalent to that obtained for mtDNA isolated by traditional procedures.

The efficacy of a large number of detergents, agarose concentrations, embedding conditions, embedding temperatures and electrophoretic conditions were examined to determine the optimal conditions for solid phase extraction. Possible uses for the new procedure are discussed.

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DOI

10.25777/sv11-rh15

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