Date of Award

Fall 2002

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

R. James Swanson

Committee Director

Mahmood M. Morshedi

Committee Member

Keith Carson

Call Number for Print

Special Collections LD4331.B47 F67 2002

Abstract

This study showed that fresh and frozen-thawed mouse ovaries would maintain viable primordial or primary follicles post-autologous transplantation. Furthermore, with appropriate hormonal stimulation, these early-stage follicles were shown to develop into Graafian follicles containing a metaphase II antral ovum. Partially sliced ovaries were transplanted into the abdominal cavity of naive, outbred, CD1, white mice. Hormonal stimulation with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) one week after the autologous transplantation stimulated variable degrees of follicle and ovum maturation within the ovarian tissue. There were no major morphological differences observed between the four groups, three treatment and one control, in thecal cell, basement membrane, or granulosa cell structure at the light microscopic (LM) level. Histologically normal follicles developed from non-frozen, frozen-thawed, and non-transplanted ovarian tissue groups. The cryopreservation and transplantation procedure in non-frozen and frozen-thawed ovarian tissue adversely affected the developmental rate prophase I oocytes to metaphase II oocytes.

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DOI

10.25777/cr81-cs42

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