Date of Award

Spring 1989

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

Ralph W. Stevens, III

Committee Member

Lloyd Wolfinbarger, Jr.

Committee Member

R. James Swanson

Call Number for Print

Special Collections LD4331.B46 H35

Abstract

The purpose was to determine whether dogs and hamsters contain the same sperm-specific protamine and testis specific proteins (TP) as other eutherian mammals previously studied. The proteins (protamine and TPs) were isolated from spermatids and mature spermatozoa, and were subjected to electrophoresis in an acid-urea, polyacrylamide gel system according to the procedure of Panyim and Chalkley (1962). The results indicated that, regardless of the anatomical location of sperm acquisition, acid precipitation technique, or denaturing agents used, epididymal and testicular samples from both the dog and hamster contained protamine in elongated spermatids and mature spermatozoa. The protamines from dogs and hamsters co-migrated with the highly characterized mouse protamine. When the RfH4 and charge to mass ratios where calculated for the dog and hamster they closely mirrored the values of the mouse. The Rftt4 value for these animals was 2.01 ±0.04. The charge to mass ratios for the dog and hamster were 4.37 and 4.38, respectively. In this study protamines were isolated from the elongated spermatids and mature spermatozoa of the dog and hamster. The presence of TP proteins could not be confirmed.

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DOI

10.25777/rh8v-1m09

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