Date of Award

Spring 1980

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Program/Concentration

Biology

Committee Director

James C. Johnson

Committee Member

Samuel Merritt

Committee Member

John R. Richardson

Call Number for Print

Special Collections LD4331.B46 N37

Abstract

The presence of large numbers of enteric viruses in wastewaters has resulted in concern regarding their significance and consequences for humans. Assessments of relative significance or consequences may most accurately be made following precise measurements of the range of virus types and amounts in wastewaters and sludges and after epidemiological considerations. In this study, results are presented pertaining to enteric virus isolation, concentration, and enumeration from primary wastewater sludges.

As an integral part of a comparison of isolation procedures, several anionic surfactants were examined for extraction of enteric viruses from primary wastewater sludges. Plaquing efficiencies for poliovirus type 2 and coxsackievirus B2 on several common cell lines were determined. A new and efficient multiple extraction-concentration procedure for the isolation and enumeration of viruses indigenous to primary wastewater sludges is described.

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DOI

10.25777/qa05-2g73

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