Date of Award

Spring 1980

Document Type


Degree Name

Master of Science (MS)


Biological Sciences



Committee Director

James C. Johnson

Committee Member

Samuel Merritt

Committee Member

John R. Richardson

Call Number for Print

Special Collections LD4331.B46 N37


The presence of large numbers of enteric viruses in wastewaters has resulted in concern regarding their significance and consequences for humans. Assessments of relative significance or consequences may most accurately be made following precise measurements of the range of virus types and amounts in wastewaters and sludges and after epidemiological considerations. In this study, results are presented pertaining to enteric virus isolation, concentration, and enumeration from primary wastewater sludges.

As an integral part of a comparison of isolation procedures, several anionic surfactants were examined for extraction of enteric viruses from primary wastewater sludges. Plaquing efficiencies for poliovirus type 2 and coxsackievirus B2 on several common cell lines were determined. A new and efficient multiple extraction-concentration procedure for the isolation and enumeration of viruses indigenous to primary wastewater sludges is described.


In Copyright. URI: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).