Date of Award

Summer 1979

Document Type


Degree Name

Master of Science (MS)


Biological Sciences



Committee Director

Edgar F. Stillwell

Committee Director

George L. Wright, Jr.

Committee Member

Lloyd Wolfinbarger


A two-dimensional polyacrylamide gel electrophoretic technique was developed for the detection of unique tumor-associated proteins in urine of patients with transitional cell carcinoma (TCC) of the urinary bladder. This technique yielded high resolution of polypeptides in the urine of cancer patients. Urine was chosen as a source of tumor-associated components because the location of the tum or should release already solublized tum or substances in to the urine.

Urinary proteins were concentrated and then analyzed by the two - dimensional electrophoretic technique using isoelectric focusing in the first dimension and sodium dodecylsulfate - polyacrylamide gel electrophoresis in the second dimension. Ten transitional cell carcinoma patients, ten renal cell carcinoma patients, two benign bladder patients, two benign kidney patients, and seven normal donors were included in this study. Urinary proteins that were associated with transitional cell carcinoma and not found in normal urine or urine from patients with benign diseases are described.


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