Document Type

Article

Publication Date

2012

DOI

10.1093/jhered/esr139

Publication Title

Journal of Heredity

Volume

103

Issue

3

Pages

459-464

Abstract

Genetic analyses using ancient DNA from Pleistocene and early Holocene fossils have largely relied on mitochondrial DNA (mtDNA) sequences. Among woolly mammoths, Mammuthus primigenius, mtDNA analyses have identified 2 distinct clades (I and II) that diverged 1-2 Ma. Here, we establish that microsatellite markers can be effective on Pleistocene samples, successfully genotyping woolly mammoth specimens at 2 loci. Although significant differentiation at the 2 microsatellite loci was not detected between 16 clade I and 4 clade II woolly mammoths, our results demonstrate that the nuclear population structure of Pleistocene species can be examined using fast-evolving nuclear microsatellite markers.

Comments

Web of Science: "Free full-text from publisher."

Original Publication Citation

Ishida, Y., Roca, A. L., Fratpietro, S., & Greenwood, A. D. (2012). Successful genotyping of microsatellites in the woolly mammoth. Journal of Heredity, 103(3), 459-464. doi:10.1093/jhered/esr139

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