Document Type

Article

Publication Date

2021

DOI

10.3389/fphys.2021.710026

Publication Title

Frontiers in Physiology

Volume

12

Pages

710026 (1-12)

Abstract

Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations. Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ'17O, defined as the residual from the tight linear correlation that naturally exists between δ17O and δ18O values. Importantly, Δ'17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance. We evaluated the effects of changes in metabolic rate and water intake on Δ'17O values of captive rufous-collared sparrows (Zonotrichia capensis) and two invertivorous passerine species in the genus Cinclodes from the field. As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis, causing a consistent change in Δ'17O. Measurement of Δ'17O also provides an estimate of the δ18O composition of ingested pre-formed (drinking/food) water. Estimated δ18O values of drinking/food water for captive Z. capensis were ~ −11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ18O values of drinking/food water ingested by wild-caught Cinclodeswere similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds.

Rights

© 2021 Sabat, Newsome, Pinochet, Nespolo, Sanchez-Hernandez, Maldonado, Gerson, Sharp and Whiteman.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Data Availability

Article states: "The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation."

Corresponding author: Pablo Sabat

Original Publication Citation

Sabat, P., Newsome Seth D., Pinochet, S. ... Gerson, A., Sharp, Z.D., & Whiteman, J.P. (2021) Triple oxygen isotope measurements (Δ'17O) of body water reflect water intake, metabolism, and δ18O of ingested water in passerines. Frontiers in Physiology, 12, 1-12, Article 710026. https://doi.org/10.3389/fphys.2021.710026

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