Document Type
Article
Publication Date
2025
DOI
10.3390/plants14142169
Publication Title
Plants
Volume
14
Issue
14
Pages
2169
Abstract
Soybean (Glycine max) peptide lunasin exhibits significant cancer-preventive, antioxidant, and hypocholesterolemic effects. This study aimed to identify quantitative trait nucleotides (QTNs) associated with lunasin content and to annotate the candidate genes in the soybean genome. The mapping panel of 144 accessions was gathered from the USDA Soybean Germplasm Collection, encompassing diverse geographical origins and genetic backgrounds, and was genotyped using SoySNP50K iSelect Beadchips. The lunasin content in soybean seeds was measured using the enzyme-linked immunosorbent assay (ELISA) method, with lipid-adjusted soybean flour prepared from seeds obtained from the Germplasm Resource Information Network (GRIN) of USDA-ARS in 2003 and from North Carolina in 2021, respectively. QTNs significantly related to lunasin content in soybean seeds were detected on 15 chromosomes, with LOD scores greater than 3.0, explaining various phenotypic variations identified using the R package mrMLM (v4.0). Significant QTNs on chromosomes 3, 13, 16, 18, and 20 were consistently identified across multiple models as being significantly associated with soybean lunasin content, based on assessment data from two years. Twenty-nine candidate genes were found, with 12 identified in seeds from 2003 and 17 from 2021. Our study is an important effort to understand the genetic basis and functional genes for lunasin production in soybean seeds.
Rights
© 2025 The Authors.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) License.
Data Availability
Article states: "The original contributions presented in this study are included in the article."
Original Publication Citation
Locklear, R., Kusumah, J., Rashad, L., Lugaro, F., Viera, S., Kipyego, N., Kipkosgei, F., Jerop, D., Jacquet, S., Kassem, M. A., Yuan, J., de Mejia, E., & Mian, R. (2025). Multi-locus GWAS mapping and candidate gene analysis of anticancer peptide lunasin in soybean (Glycine max L. Merr.). Plants, 14(14), Article 2169. https://doi.org/10.3390/plants14142169
Repository Citation
Locklear, Rikki; Kusumah, Jennifer; Rashad, Layla; Lugaro, Felicia; Viera, Sonia; Kipyego, Nathan; Kipkosgei, Faith; Jerop, Daisy; Jacquet, Shirley; Kassem, Mythy Addelmajid; Yuan, Jiazheng; Mejia, Elvira de; and Mian, Rouf, "Multi-Locas GWAS Mapping and Candidate Gene Analysis of Anticancer Peptide Lunasin in Soybean (Glycine max L. Merr)" (2025). Biological Sciences Faculty Publications. 653.
https://digitalcommons.odu.edu/biology_fac_pubs/653
ORCID
0000-0002-8937-4361 (Rashad)
Included in
Agricultural Science Commons, Amino Acids, Peptides, and Proteins Commons, Genetics Commons, Oncology Commons