Document Type

Article

Publication Date

2026

DOI

10.1038/s41598-026-56104-5

Publication Title

Scientific Reports

Pages

21 pp.

Abstract

Platanthera is among the most diverse genera of orchids in north temperate regions, with high species diversity in Asia and North America. Despite many ecological studies in this group, little is known about its genomic diversity. Here, we report the newly annotated chloroplast genomes from six species in subgenus Limnorchis from North America and compare them with plastomes published for Platanthera species from Asia and Europe. We found that the plastomes of species in subg. Limnorchis are among the smallest reported for Platanthera thus far. While major structural differences were not detected in the newly sequenced species, ndh genes were frequently absent or contained internal stop codons, suggesting their lack of functionality in subg. Limnorchis but not in other species of Platanthera. Numerous SSRs, primarily A/T repeats, were detected across the six species, and patterns of codon usage varied little among species. Phylogenetic analysis indicated strong support for monophyly of recognized subgenera, as well as consistency with patterns suggested from nuclear loci in subg. Limnorchis. The new plastome sequences contribute to greater understanding of genome variation in relation to diversification of Platanthera and will be useful for phylogenetic analysis.

Rights

© The Authors 2026.

This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if you modified the licensed material. You do not have permission under this license to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Data Availability

Article states: "Voucher specimens of all sequenced samples are deposited in herbaria (see Table 1). Annotated genome sequences are deposited in Genbank (https://www.ncbi.nlm.nih.gov/nuccore/)."

Original Publication Citation

Wallace, L. E., Batalla, M. I., & Maisonave, M. (2026). Comparative analysis of six new chloroplast genomes in Platanthera (Orchidaceae) enhances understanding of its diversification. Scientific Reports. Advance online publication. https://doi.org/10.1038/s41598-026-56104-5

ORCID

0000-0001-6665-5454 (Wallace), 0009-0002-7518-9883 (Maisonave)

Additional Files
41598_2026_56104_MOESM1_ESM.docx (2933 kB)
Supplementary Information
Download

Share

Article Location

 
COinS