Date of Award

Summer 1980

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

James H. Yuan

Committee Member

Thomas O. Stiz

Committee Member

Edward Wortham

Call Number for Print

Special Collections LD4331.C45G38


A comprehensive biochemical screening procedure for the evaluation of seminal plasma constituents is needed which, when used in conjunction with semen analysis, can provide the physician with both qualitative and quantitative information regarding the fertility status of an individual.

High resolution two-dimensional electrophoresis is a technique whereby proteins in a complex mixture such as a biological fluid are separated in one dimension by molecular charge and in the second dimension by molecular mass. It has been the purpose of this investigation to adapt and evaluate this technique for the analysis of human seminal plasma.

Our efforts have shown that this technique is potentially useful for use as an andrological screening procedure. Distinct protein patterns have been produced containing up to two hundred detectable components with several proteins having been either tentatively or positively identified.


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