Date of Award

Summer 1984

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

Patricia A. Pleban

Committee Member

Daniel Sonenshine

Committee Member

John Van Norman

Call Number for Print

Special Collections LD4331.C45S33


A total of 44 Long-Evans rats received 5.7 g/kg tri-O-cresyl phosphate (TOCP) orally every 3 days for 6 days. A second group of 13 rats received 4.6 g/kg orally every 3 days for 6 days. Animals were administered atropine sulfate (10 mg/kg) intraperitoneally every 6 hours or as needed to alleviate cholinergic symptoms. Ten control animals were given corn oil orally. Eight rats (18%) survived the 5.7 g/kg dose regimen and 5 rats (38%) survived the 4.6 g/kg dose regimen. Surviving rats were killed at 30-33 days post-dose. Signs of delayed neuropathy included ataxia and motor impairment.

The CNP activity was monitored in tissue preparations of red blood cell (RBC) ghosts, brain and spinal cord from TOCP treated and control animals. The CNP activity values for dosed and control spinal cord were 246 and 299 nmoles/min/mg protein, respectively. The CNP activities were significantly different in spinal cord preparations of dosed and control animals at the 95% confidence level (P<0.05), as determined by the two-tailed t test. However, CNP activities for brain and RBC ghosts were not significantly different.


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