Date of Award

Summer 1986

Document Type


Degree Name

Master of Science (MS)


Chemistry & Biochemistry



Committee Director

John D. Van Norman

Committee Member

Kenneth G. Brown

Committee Member

Myung H. Kim

Call Number for Print

Special Collections LD4331.C45W34


Numerous investigations of bilirubin have been reported in the literature. Correlation among these investigations can not be made because of multiple behavioral changes characteristic of the solubilized pigment. This research was initiated to evaluate bilirubin and its associated isomers in identical chemical environments.

Two different solvent systems were experimentally selected to study chemical behavior of bilirubin and occurring photoisomers. A ternary solution consisting of 1:3:1 chloroform:methanol:water was used exclusively in visible spectrophotometric analyses. Also, a pH 10.00 buffer was utilized. This latter system has successfully been used in spectrophotometric and voltammetric work.

Four different photochemical processes have been detected spectrophotometrically, one of which has not been previously reported. In addition, voltammetric potentials of photobilirubin have been determined and compared with bilirubin and biliverdin. This was made possible by a novel extraction procedure permitting investigation of photobilirubin by itself.


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