Document Type
Article
Publication Date
2015
DOI
10.1515/bimo-2015-0004
Publication Title
Biomonitoring
Volume
2
Issue
1
Pages
35-42
Abstract
The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of 8-oxodGuo levels in sperm were related to the percentages of DNA fragmentation measured by both the TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039). Sperm vitality, motility and morphology from all of the participants exhibited a weak correlation with the levels of 8-oxodGuo and the percentages of DNA fragmentation. Semen quality parameters may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Semen quality parameters may be insufficient to monitor sperm DNA fragmentation and oxidative damage. DNA damage in sperm is recommended to be included in routine measurements.
Original Publication Citation
Jeng, H. A., Li, R.-N., & Lin, W.-Y. (2015). Are semen quality parameters sufficient for biomonitoring spermatozoa DNA integrity and oxidatively damaged DNA. Biomonitoring, 2(1), 35-42. doi:10.1515/bimo-2015-0004
Repository Citation
Jeng, Hueiwang Anna; Li, Ruei-Nian; and Lin, Wen-Yi, "Are Semen Quality Parameters Sufficient for Biomonitoring Spermatozoa DNA Integrity and Oxidatively Damaged DNA" (2015). Community & Environmental Health Faculty Publications. 62.
https://digitalcommons.odu.edu/commhealth_fac_pubs/62
Comments
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
© 2015 Hueiwang Anna Jeng et al. licensee De Gruyter Open.