Document Type

Article

Publication Date

2014

DOI

10.1186/1471-2105-15-s8-s3

Publication Title

BMC Bioinformatics

Volume

15

Pages

1-8

Conference Name

Third IEEE International Conference on Computational Advances in Bio and Medical Sciences (ICCABS 2013)

Abstract

Background: Secondary structures prediction of proteins is important to many protein structure modeling applications. Correct prediction of secondary structures can significantly reduce the degrees of freedom in protein tertiary structure modeling and therefore reduces the difficulty of obtaining high resolution 3D models.

Methods: In this work, we investigate a template-based approach to enhance 8-state secondary structure prediction accuracy. We construct structural templates from known protein structures with certain sequence similarity. The structural templates are then incorporated as features with sequence and evolutionary information to train two-stage neural networks. In case of structural templates absence, heuristic structural information is incorporated instead.

Results: After applying the template-based 8-state secondary structure prediction method, the 7-fold cross-validated Q8 accuracy is 78.85%. Even templates from structures with only 20% ~ 30% sequence similarity can help improve the 8-state prediction accuracy. More importantly, when good templates are available, the prediction accuracy of less frequent secondary structures, such as 3-10 helices, turns, and bends, are highly improved, which are useful for practical applications.

Conclusions: Our computational results show that the templates containing structural information are effective features to enhance 8-state secondary structure predictions. Our prediction algorithm is implemented on a web server named "C8-SCORPION" available at: http://hpcr.cs.odu.edu/c8scorpion.

Comments

Creative Commons Attribution License

http://creativecommons.org/licenses/by/2.0

Original Publication Citation

Yaseen, A., & Li, Y.H. (2014). Template-based c8-scorpion: A protein 8-state secondary structure prediction method using structural information and context-based features. BMC Bioinformatics, 15. doi: 10.1186/1471-2105-15-s8-s3

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