Document Type

Conference Paper

Publication Date

2019

DOI

10.1117/12.2514299

Publication Title

Health Monitoring of Structural and Biological Systems XIII

Volume

10972

Pages

1-9

Conference Name

Health Monitoring of Structural and Biological Systems XIII, March 4-7, 2019

Abstract

Optical stretcher is a tool in which two counter-propagating, slightly diverging, and identical laser beams are used to trap and axially stretch microparticles in the path of light. In this work, we utilized the dual-beam optical stretcher setup to trap and stretch human embryonic kidney (HEK) cells and mammalian breast cancer (MBC) cells. Experiments were performed by exposing the HEK cells to counter-propagating laser beams for 30 seconds at powers ranging from 100 mW to 561 mW. It was observed that the percentage of cell deformation increased from 16.7% at 100 mW to 40.5% at 561 mW optical power. The MBC cells exhibited significantly higher cell stretching compared to HEK cells at the same power (80 mW). Moreover, the minimum trapping power in HEK cells was 80.5mW as compared to 65.2mW in MBC cells. This study provides useful insights into the characterization of cytoskeletal elasticity in different cell types based on non-contact optical cell stretching.

Comments

Copyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.

Original Publication Citation

Aotuo Dong, Balaadithya Uppalapati, Md. Shariful Islam, Brandon Gibbs, Ganesan Kamatchi, Sacharia Albin, and Makarand Deo "Non-contact trapping and stretching of biological cells using dual-beam optical stretcher on microfluidic platform", Proc. SPIE 10972, Health Monitoring of Structural and Biological Systems XIII, 109722A (1 April 2019); https://doi.org/10.1117/12.2514299.

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