Document Type

Article

Publication Date

2022

DOI

10.1016/j.bioelechem.2021.107980

Publication Title

Bioelectrochemistry

Volume

144

Pages

107980 (1-11)

Abstract

Tendon injuries are a common athletic injury that have been increasing in prevalence. While there are current clinical treatments for tendon injuries, they have relatively long recovery times and often do not restore native function of the tendon. In the current study, gene electrotransfer (GET) parameters for delivery to the skin were optimized with monophasic and biphasic pulses with reporter and effector genes towards optimizing underlying tendon healing. Tissue twitching and damage, as well as gene expression and distribution were evaluated. Bioprinted collagen scaffolds, mimicking healthy tendon structure were then implanted subcutaneously for biocompatibility and angiogenesis analyses when combined with GET to accelerate healing. GET of human fibroblast FGF2 significantly increased angiogenesis and biocompatibility of the bioprinted implants when compared to implant only sites. The combination of bioprinted collagen fibers and angiogenic GET therapy may lead to better graft biocompatibility in tendon repair.

Comments

© 2021 The Authors.

This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.

Original Publication Citation

Boye, C., Christensen, K., Asadipour, K., DeClemente, S., Francis, M., & Bulysheva, A. (2022). Gene electrotransfer of FGF2 enhances collagen scaffold biocompatibility. Bioelectrochemistry, 144, 1-11, Article 107980. https://doi.org/10.1016/j.bioelechem.2021.107980

ORCID

0000-0002-7287-9371 (Bulysheva)

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