Document Type
Article
Publication Date
2016
DOI
10.14814/phy2.12890
Publication Title
Physiological Reports
Volume
4
Issue
15
Pages
e12890 (1-9)
Abstract
The study aims to characterize age-associated changes in skeletal muscle bioenergetics by evaluating the response to ischemia-reperfusion in the skeletal muscle of the Goto-Kakizaki (GK) rats, a rat model of non-obese type 2 diabetes (T2D). 31P magnetic resonance spectroscopy (MRS) and blood oxygen level-dependent (BOLD) MRI was performed on the hindlimb of young (12 weeks) and adult (20 weeks) GK and Wistar (control) rats. 31P-MRS and BOLD-MRI data were acquired continuously during an ischemia and reperfusion protocol to quantify changes in phosphate metabolites and muscle oxygenation. The time constant of phosphocreatine recovery, an index of mitochondrial oxidative capacity, was not statistically different between GK rats (60.8 ± 13.9 sec in young group, 83.7 ± 13.0 sec in adult group) and their age-matched controls (62.4 ± 11.6 sec in young group, 77.5 ± 7.1 sec in adult group). During ischemia, baseline-normalized BOLD-MRI signal was significantly lower in GK rats than in their age-matched controls. These results suggest that insulin resistance leads to alterations in tissue metabolism without impaired mitochondrial oxidative capacity in GK rats. © 2016 The Authors.
Original Publication Citation
Liu, Y., Mei, X., Li, J., Lai, N., & Yu, X. (2016). Mitochondrial function assessed by 31P MRS and BOLD MRI in non-obese type 2 diabetic rats. Physiological Reports, 4(15), e12890. doi:10.14814/phy2.12890
Repository Citation
Liu, Yuchi; Mei, Xunbai; Li, Jielei; Lai, Nicola; and Yu, Xin, "Mitochondrial Function Assessed by 31P MRS and BOLD MRI in Non-Obese Type 2 Diabetic Rats" (2016). Electrical & Computer Engineering Faculty Publications. 80.
https://digitalcommons.odu.edu/ece_fac_pubs/80
ORCID
0000-0002-3436-289X (Lai)
Included in
Biochemical Phenomena, Metabolism, and Nutrition Commons, Biochemistry Commons, Endocrinology, Diabetes, and Metabolism Commons, Systems and Integrative Physiology Commons, Systems Biology Commons
Comments
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license.
https://creativecommons.org/licenses/by/3.0/