3D Bioprinted Structures from Cells of Non-Epithelial Mesodermal and Endodermal Lineage Using a Custom Accessible 3D Bioprinting Platform
ORCID
https://orcid.org/0000-0002-1289-5302
College
College of Engineering & Technology (Batten)
Department
Biomedical Engineering
Graduate Level
Doctoral
Graduate Program/Concentration
Biomedical Engineering
Publication Date
2022
DOI
10.25883/0dbx-ze17
Abstract
Prior work within our lab has demonstrated the ability to print both murine and human mammary organoids and tumoroids in vitro that can also be reliably transplanted into a murine host for translational studies. Peripherally, this bioprinting system has also been used for 3D printing neurons, stem cells, cancer cells, and a primary cell line rich with fibroblasts, but each of these efforts were with cells of ectodermal lineage. Thus, the system's capacity for use on cells of other origins had been untested. To address this, we have now developed protocols for cells of endodermal and non-epithelial mesodermal/mesenchymal lineage. In this work, we find that we can produce reliable organoids, tumoroids, and other in vitro structures from them, thus expanding the functional range of our open 3D bioprinting platform. Therefore, we demonstrate that our system is versatile for adaptation to multiple cellular systems and can be applied to the work of labs that wish to study development and pathologies in other organ systems
Keywords
Bioprinting, Organoid, Cancer, Tumoroid, Murine, Human, Translation, Implantation, Ectoderm, Mesoderm, Endoderm, Epithelial, Non-Epithelial
Disciplines
Biological Engineering | Biology and Biomimetic Materials | Biomaterials | Biomedical Engineering and Bioengineering | Biotechnology | Integrative Biology | Medical Pathology | Molecular, Cellular, and Tissue Engineering | Other Biochemistry, Biophysics, and Structural Biology | Other Biomedical Engineering and Bioengineering | Systems and Integrative Engineering | Translational Medical Research
Files
Recommended Citation
Palmer, Xavier-Lewis; Berkin, Annette; Sachs, Patrick C.; and Bruno, Robert D., "3D Bioprinted Structures from Cells of Non-Epithelial Mesodermal and Endodermal Lineage Using a Custom Accessible 3D Bioprinting Platform" (2022). College of Engineering & Technology (Batten) Posters. 2.
https://digitalcommons.odu.edu/gradposters2022_engineering/2