ITGA6, NOS2, PI3 and POSTN Suppress Glioblastoma Multiforme Induced Epilepsy
Abstract/Description/Artist Statement
ITGA6, NOS2, PI3 and POSTN Suppress Glioblastoma Multiforme Induced Epilepsy
Andrei Sanda, Ambrosio Valencia-Romero PhD, Liss Romero Fandiño MSc, Alberto Musto MD-PhD
Glioblastoma Multiforme (GBM) is an aggressive brain tumor with a poor prognosis. Previous studies have identified multiple genes in the peritumoral zone (PTZ), but there is limited research that directly links specific genes to epileptogenesis in GBM. In this project, we sought to determine if there are genes in the PTZ of GBM that correlate with epilepsy. 481 genes across 19 patients with or without a history of seizures were analyzed using in-situ hybridization (ISH) data on the publicly available Ivy Glioblastoma Atlas Project (GAP) database. The genes were examined for their presence at different distances from the tumor boundary. Using a phi coefficient and Fisher’s exact test analysis, a network of genes was created to elicit relationships between individual genes, genes versus epilepsy, and genes versus distance. The gene network highlights several genes that are present together in the PTZ. CD44, IGFBP2, and PDPN were negatively correlated with distance, and ITGA6, NOS2, PI3, and POSTN were negatively correlated with epilepsy. In addition, preliminary data from MRI images suggests that there is an inverse association between the presence of epilepsy and the magnitude of peritumoral edema (r= -0.23). We hypothesize the existence of an underlying mechanism that these genes utilize to suppress epilepsy in GBM.
Keywords: Glioblastoma Multiforme, Epilepsy, Genes, Network, Edema
ITGA6, NOS2, PI3 and POSTN Suppress Glioblastoma Multiforme Induced Epilepsy
ITGA6, NOS2, PI3 and POSTN Suppress Glioblastoma Multiforme Induced Epilepsy
Andrei Sanda, Ambrosio Valencia-Romero PhD, Liss Romero Fandiño MSc, Alberto Musto MD-PhD
Glioblastoma Multiforme (GBM) is an aggressive brain tumor with a poor prognosis. Previous studies have identified multiple genes in the peritumoral zone (PTZ), but there is limited research that directly links specific genes to epileptogenesis in GBM. In this project, we sought to determine if there are genes in the PTZ of GBM that correlate with epilepsy. 481 genes across 19 patients with or without a history of seizures were analyzed using in-situ hybridization (ISH) data on the publicly available Ivy Glioblastoma Atlas Project (GAP) database. The genes were examined for their presence at different distances from the tumor boundary. Using a phi coefficient and Fisher’s exact test analysis, a network of genes was created to elicit relationships between individual genes, genes versus epilepsy, and genes versus distance. The gene network highlights several genes that are present together in the PTZ. CD44, IGFBP2, and PDPN were negatively correlated with distance, and ITGA6, NOS2, PI3, and POSTN were negatively correlated with epilepsy. In addition, preliminary data from MRI images suggests that there is an inverse association between the presence of epilepsy and the magnitude of peritumoral edema (r= -0.23). We hypothesize the existence of an underlying mechanism that these genes utilize to suppress epilepsy in GBM.
Keywords: Glioblastoma Multiforme, Epilepsy, Genes, Network, Edema