Document Type

Article

Publication Date

2004

DOI

10.1186/1471-2105-5-26

Publication Title

BMC Bioinformatics

Volume

5

Pages

26 (9 pages)

Abstract

Background: Recent technological advances in mass spectrometry pose challenges in computational mathematics and statistics to process the mass spectral data into predictive models with clinical and biological significance. We discuss several classification-based approaches to finding protein biomarker candidates using protein profiles obtained via mass spectrometry, and we assess their statistical significance. Our overall goal is to implicate peaks that have a high likelihood of being biologically linked to a given disease state, and thus to narrow the search for biomarker candidates.

Results: Thorough cross-validation studies and randomization tests are performed on a prostate cancer dataset with over 300 patients, obtained at the Eastern Virginia Medical School using SELDI-TOF mass spectrometry. We obtain average classification accuracies of 87% on a four-group classification problem using a two-stage linear SVM-based procedure and just 13 peaks, with other methods performing comparably.

Conclusions: Modern feature selection and classification methods are powerful techniques for both the identification of biomarker candidates and the related problem of building predictive models from protein mass spectrometric profiles. Cross-validation and randomization are essential tools that must be performed carefully in order not to bias the results unfairly. However, only a biological validation and identification of the underlying proteins will ultimately confirm the actual value and power of any computational predictions.

Comments

This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

(https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-5-26)

Original Publication Citation

Wagner, M., Naik, D. N., Pothen, A., Kasukurti, S., Devineni, R. R., Adam, B. L., . . . Wright, G. L. (2004). Computational protein biomarker prediction: A case study for prostate cancer. BMC Bioinformatics, 5, 26. doi:10.1186/1471-2105-5-26

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