ORCID
0009-0000-2499-4714 (Desai)
Document Type
Article
Publication Date
2025
DOI
10.1002/acr.25658
Publication Title
Arthritis Care & Research
Volume
Advance online publication
Pages
1-9
Abstract
Objective
Ultrasound (US) has been proposed as a potential tool for assessing skin fibrosis in systemic sclerosis (SSc). However, a large-scale comparison of US-based assessment with histologic markers of skin fibrosis has not been reported. We evaluated US-based skin assessments for their face validity (differentiation between involved SSc and healthy control [HC] skin), construct validity (comparison to modified Rodnan skin score [mRSS]), and criterion validity (comparison to histologic and gene expression fibrosis markers).
Methods
Twenty HCs and 52 patients with SSc underwent clinical and US assessment followed by a forearm skin biopsy. Predefined areas were assessed on the finger, hand, and forearm bilaterally. mRSS, US, histologic and molecular (reverse transcription quantitative polymerase chain reaction) evaluations were performed by blinded, independent assessors. Dermal thickness, echogenicity, and elastography were assessed using a high-frequency GE LOGIQ P9 US machine.
Results
Except in the hand area, the US variables could not differentiate between HC and clinically affected SSc skin (face validity). There was only a weak to moderate correlation between US-based measurements and mRSS in the hand and finger areas (construct validity). US-based thickness showed moderate correlation with histologic thickness (p = 0.43; P = 0.002) but no statistically significant correlations with other histologic or gene expression markers of fibrosis in patients with SSc (criterion validity).
Conclusion
High-frequency US assessment of SSc skin does not show consistent and strong face, construct, or criterion validity. The role of this assessment tool remains limited, underscoring the need for further development of a quantitative and accurate tool for assessing SSc skin fibrosis.
Rights
© 2025 The Authors
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Original Publication Citation
Desai, R., Tan, F., Wu, M. H., Browning, J. L., Theodore, S., Zhang, M., Skaug, B., Chawla, H. S., Singh, M., Aglyamov, S., Larin, K. V., Mayes, M., & Assassi, S. (2025). Assessment of skin in patients with systemic sclerosis using high-frequency ultrasound and shear wave elastography: A comparative study with histology, molecular, and clinical parameters. Arthritis Care & Research. Advance online publication. https://doi.org/10.1002/acr.25658
Repository Citation
Desai, R., Tan, F., Wu, M. H., Browning, J. L., Theodore, S., Zhang, M., Skaug, B., Chawla, H. S., Singh, M., Aglyamov, S., Larin, K. V., Mayes, M., & Assassi, S. (2025). Assessment of skin in patients with systemic sclerosis using high-frequency ultrasound and shear wave elastography: A comparative study with histology, molecular, and clinical parameters. Arthritis Care & Research. Advance online publication. https://doi.org/10.1002/acr.25658
Disclosure Form
acr25658-sup-0002-supinfo.docx (2490 kB)
Appendix S1: Supplementary Information