Document Type

Article

Publication Date

11-2004

DOI

10.1128/aem.70.11.6495-6500.2004

Publication Title

Applied and Environmental Microbiology

Volume

70

Issue

11

Pages

6495-6500

Abstract

It is well established that cyst-forming phytoplankton species are transported in ships' ballast tanks. However, there is increasing evidence that other phytoplankton species which do not encyst are also capable of surviving ballast transit. These species have alternative modes of nutrition (hetero- or mixotrophy) and/or are able to survive long-term darkness. In our studies of no-ballast-on-board vessels arriving in the Great Lakes, we tested for the presence of the harmful algal bloom species Aureococcus anophagefferens (brown tide) in residual (i.e., unpumpable) ballast water using methods based on the PCR. During 2001, the brown tide organism was detected in 7 of 18 ballast water tanks in commercial ships following transit from foreign ports. Furthermore, it was detected after 10 days of ballast tank confinement during a vessel transit in the Great Lakes, a significant result given the large disparity between the salinity tolerance for active growth of Aureococcus (>22 ppt) and the low salinity of the residual ballast water (similar to 2 ppt). We also investigated the potential for smaller, recreational vessels to transport and distribute Aureococcus. During the summer of 2002, 11 trailered boats from the inland bays of Delaware and coastal bays of Maryland were sampled. Brown tide was detected in the bilge water in the bottoms of eight boats, as well as in one live-well sample. Commercial ships and small recreational boats are therefore implicated as potential vectors for long-distance transport and local-scale dispersal of Aureococcus.

Original Publication Citation

Doblin, M.A., Popels, L.C., Coyne, K.J., Hutchins, D.A., Cary, S.C., & Dobbs, F.C. (2004). Transport of the harmful bloom alga Aureococcus anophagefferens by oceangoing ships and coastal boats. Applied and Environmental Microbiology, 70(11), 6495-6500. doi: 10.1128/aem.70.11.6495-6500.2004

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