ORCID

0000-0002-6751-6250 (Huang), 0000-0001-8281-3184 (Bochdansky)

Document Type

Article

Publication Date

2025

DOI

10.3389/fmars.2025.1539828

Publication Title

Frontiers in Marine Science

Volume

12

Pages

1539828

Abstract

A variety of imaging systems are in use in oceanographic surveys, and the opto-mechanical configurations have become highly sophisticated. However, much less consideration has been given to the accurate reconstruction of imaging data. To improve reconstruction of particles captured by Focused Shadowgraph Imaging (FoSI)—a system that excels at visualizing low-optical-density objects, we developed a novel object detection algorithm to process images with a resolution of ~ 12 μm per pixel. Suggested improvements to conventional edge-detection methods are relatively simple and time-efficient, and more accurately render the sizes and shapes of small particles ranging from 24 to 500 μm. In addition, we introduce a gradient of neutral density filters as a part of the protocol serving to calibrate recorded gray levels and thus determine the absolute values of detection thresholds. Set to intermediate detection threshold levels, particle numbers were highly correlated with beam attenuation (cp) measured independently. The utility of our method was underscored by its ability to remove imperfections (dirt, scratches and uneven illumination), and by capturing the transparent particle features such as found in gelatinous plankton, marine snow and a portion of the oceanic gel phase.

Rights

© 2025 Huang and Bochdansky.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0). The use, distribution or reproduction in other forums is permitted, provided the original authors and the copyright owners are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Data Availability

Article states: "The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation."

Original Publication Citation

Huang, H., & Bochdansky, A. B. (2025). Optimizing an image analysis protocol for ocean particles in focused shadowgraph imaging systems. Frontiers in Marine Science, 12, 1-19, Article 1539828. https://doi.org/10.3389/fmars.2025.1539828

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