Document Type
Article
Publication Date
5-1986
DOI
10.1016/S0006-3495(86)83729-8
Publication Title
Biophysical review
Volume
49
Issue
5
Pages
1009-1015
Abstract
Samples of 90% enriched 57Fe hemoglobin and its isolated subunits have been prepared. Mössbauer spectroscopic measurements have been made on three such samples. Sample one contained contributions of oxyhemoglobin, deoxyhemoglobin, and carbonmonoxyhemoglobin. This sample was studied from a temperature of 90 K down to 230 mK. Measurements were also made at 4.2 K using a small applied magnetic field of 1.0 T. In general, the measured quadrupole splittings and isomer shifts for each component agreed with previous measurements on single component samples in the literature, and thus demonstrated that chemically enriched hemoglobin has not been altered. The second and third samples were isolated α and β subunits, respectively. We have found measurable Mössbauer spectral differences between the HbO2 sites in the α subunit sample and the β subunit sample. The measured Mössbauer spectral areas indicate that the iron ion has the largest mean-square displacement at the deoxy Hb sites as compared to that at the oxy- and carbonmonoxy Hb sites. The mean-square displacement at the HbO2 sites is the smallest.
Rights
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Copyright © 1986 The Biophysical Society. Published by Elsevier Inc. All rights reserved.
Original Publication Citation
Hoy, G. R., Cook, D. C., Berger, R. L., & Friedman, F. K. (1986). Mössbauer spectroscopic studies of hemoglobin and its isolated subunits. Biophysical Journal, 49(5), 1009-1015. https://doi.org/10.1016/S0006-3495(86)83729-8
Repository Citation
Hoy, G. R.; Cook, D. C.; Berger, R. L.; and Friedman, F. K., "Mössbauer Spectroscopic Studies of Hemoglobin and Its Isolated Subunits" (1986). Physics Faculty Publications. 984.
https://digitalcommons.odu.edu/physics_fac_pubs/984