Differential Expression of Developmental and Signaling Genes Between Normal Chondrocytes and Chondrosarcoma Cells
Description/Abstract/Artist Statement
Introduction
Resistant to both radiation and chemotherapy, chondrosarcomas can be difficult to treat. Expression levels of two developmental genes, SOX5 and SOX6 were studied in chondrosarcoma cells and compared to normal chondrocytes. The expression levels of GPR68 which is involved mediation of growth inhibition and the expression of KCNN-2 which plays a role in cellular proliferation were also studied. Chondrosarcomas are thought to arise from mesenchymal stem cells, thus it is important to study the expression levels of genes that may be involved in the process of normal chondrogenesis and proliferation.
Methods
Both chondrosarcoma cells and normal chondrocytes were cultured in monolayer and pellet (3D) cultures, followed by RNA isolations and conversion to cDNA. RT-qPCR was used to characterize the expression levels of the genes and the ∆∆Ct method to quantify fold change.
Results
SOX5, SOX6, GPR68, and KCNN-2 were found to be expressed at lower levels (-11.04, -67.04, -294.41 respectively) in the chondrosarcoma cells when compared to normal chondrocytes.
Conclusions
Lower expression of SOX6 likely influences the expression of aggrecan in chondrosarcomas. Lower levels of GPR68 implies a lack of mediation of growth inhibition in these cancer cells as well as lower levels of intracellular calcium. Less expression of KCNN-2 probably alters the membrane potential of the chondrocytes which effects the proliferation of the cells. Overall, the data suggests that when compared to normal chondrocytes there is lower expression of SOX5, SOX6, GPR68 and KCNN-2 in chondrosarcoma cells which likely has effects on their growth and proliferation.
Faculty Advisor/Mentor
Chris Osgood
Presentation Type
Oral Presentation
Disciplines
Cancer Biology | Cell Biology | Genetics
Session Title
Biological Sciences 1 Presentations
Location
Learning Commons @ Perry Library Conference Room 1310
Start Date
2-3-2018 9:00 AM
End Date
2-3-2018 10:00 AM
Differential Expression of Developmental and Signaling Genes Between Normal Chondrocytes and Chondrosarcoma Cells
Learning Commons @ Perry Library Conference Room 1310
Introduction
Resistant to both radiation and chemotherapy, chondrosarcomas can be difficult to treat. Expression levels of two developmental genes, SOX5 and SOX6 were studied in chondrosarcoma cells and compared to normal chondrocytes. The expression levels of GPR68 which is involved mediation of growth inhibition and the expression of KCNN-2 which plays a role in cellular proliferation were also studied. Chondrosarcomas are thought to arise from mesenchymal stem cells, thus it is important to study the expression levels of genes that may be involved in the process of normal chondrogenesis and proliferation.
Methods
Both chondrosarcoma cells and normal chondrocytes were cultured in monolayer and pellet (3D) cultures, followed by RNA isolations and conversion to cDNA. RT-qPCR was used to characterize the expression levels of the genes and the ∆∆Ct method to quantify fold change.
Results
SOX5, SOX6, GPR68, and KCNN-2 were found to be expressed at lower levels (-11.04, -67.04, -294.41 respectively) in the chondrosarcoma cells when compared to normal chondrocytes.
Conclusions
Lower expression of SOX6 likely influences the expression of aggrecan in chondrosarcomas. Lower levels of GPR68 implies a lack of mediation of growth inhibition in these cancer cells as well as lower levels of intracellular calcium. Less expression of KCNN-2 probably alters the membrane potential of the chondrocytes which effects the proliferation of the cells. Overall, the data suggests that when compared to normal chondrocytes there is lower expression of SOX5, SOX6, GPR68 and KCNN-2 in chondrosarcoma cells which likely has effects on their growth and proliferation.