Improving Detection of Secondary Structures within Medium Resolution Cryo-EM Density Maps
Description/Abstract/Artist Statement
Cryo-electron microscopy is a biophysical technique in production of electron density maps that are 3-dimensional images. Although atomic structures can be derived from such images at high-resolution, it is still challenging to predict structures when the resolution of the image is at medium resolutions (5-10Å). Although computational methods are available to extract protein secondary structures from a medium-resolution image, they lack sufficient accuracy for structure prediction. This work aims to improve detection of secondary structures such as helices and β-sheets as compared to previous work SSETracer. Detection of these secondary structures is an important prior step for methods that perform further analysis, such as protein sequencing.
Faculty Advisor/Mentor
Jing He
Presentation Type
Oral Presentation
Disciplines
Bioinformatics | Computer Sciences
Session Title
Biological Sciences 2 Presentations
Location
Learning Commons @ Perry Library Conference Room 1310
Start Date
2-3-2018 10:15 AM
End Date
2-3-2018 11:15 AM
Improving Detection of Secondary Structures within Medium Resolution Cryo-EM Density Maps
Learning Commons @ Perry Library Conference Room 1310
Cryo-electron microscopy is a biophysical technique in production of electron density maps that are 3-dimensional images. Although atomic structures can be derived from such images at high-resolution, it is still challenging to predict structures when the resolution of the image is at medium resolutions (5-10Å). Although computational methods are available to extract protein secondary structures from a medium-resolution image, they lack sufficient accuracy for structure prediction. This work aims to improve detection of secondary structures such as helices and β-sheets as compared to previous work SSETracer. Detection of these secondary structures is an important prior step for methods that perform further analysis, such as protein sequencing.