Synthesis of Bacteria Nanocellulose for Wound Dressing
Description/Abstract/Artist Statement
2% of the United States population suffers from chronic non-healing wounds. While 2% might not seem like a large number but that number converts to 6.7 million people and this number is increasing. Chronic wounds affect the aged population and also affects individuals with these conditions: Diabetes, cancer and other long-term medical conditions. It is estimated that chronic wound management has an annual cost of $25 billion in the US. Thus, smart bandaging can be used to better help manage chronic non healing wounds as they use sensors in wound monitoring to reduce the costs for wound treatment/management. In this work, we propose bacteria nanocellulose (BNC) as an ideal dressing material for the development of smart bandages. BNC is synthesized by the bacterium Gluconacetobacter Xylinus (G. Xylinus). G. Xylinus is cultured in 6-well culture plates and incubates for 5 days. On the fifth day, formation of a basal BNC pellicle is realized. Upon subsequent feeding with fresh media once every week for six weeks, additional pellicles were formed. The BNC pellicles are then extracted in 0.5 M NaOH at 90 ºC to denature proteins and bacteria. The synthesized BNC sheets are flexible thin film that is thermally and mechanically stable. They are also inert, biodegradable, and easy to sterilize. The development of smart bandages using BNC from G. Xylinus has the potential to provide a more cost-effective approach to wound monitoring and healing.
Faculty Advisor/Mentor
Gymama Slaughter
College Affiliation
College of Sciences
Presentation Type
Oral Presentation
Disciplines
Biomedical Engineering and Bioengineering | Medical Biotechnology
Session Title
Colleges of Sciences UG Research #2
Location
Zoom
Start Date
3-19-2022 2:15 PM
End Date
3-19-2022 3:15 PM
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Synthesis of Bacteria Nanocellulose for Wound Dressing
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2% of the United States population suffers from chronic non-healing wounds. While 2% might not seem like a large number but that number converts to 6.7 million people and this number is increasing. Chronic wounds affect the aged population and also affects individuals with these conditions: Diabetes, cancer and other long-term medical conditions. It is estimated that chronic wound management has an annual cost of $25 billion in the US. Thus, smart bandaging can be used to better help manage chronic non healing wounds as they use sensors in wound monitoring to reduce the costs for wound treatment/management. In this work, we propose bacteria nanocellulose (BNC) as an ideal dressing material for the development of smart bandages. BNC is synthesized by the bacterium Gluconacetobacter Xylinus (G. Xylinus). G. Xylinus is cultured in 6-well culture plates and incubates for 5 days. On the fifth day, formation of a basal BNC pellicle is realized. Upon subsequent feeding with fresh media once every week for six weeks, additional pellicles were formed. The BNC pellicles are then extracted in 0.5 M NaOH at 90 ºC to denature proteins and bacteria. The synthesized BNC sheets are flexible thin film that is thermally and mechanically stable. They are also inert, biodegradable, and easy to sterilize. The development of smart bandages using BNC from G. Xylinus has the potential to provide a more cost-effective approach to wound monitoring and healing.