Optimizing In-Vitro Transcription of a Coxsackievirus RNA Replication Element

Description/Abstract/Artist Statement

Picornaviruses are small RNA viruses responsible for illnesses such as hand-foot-mouth disease, poliomyelitis, and the common cold. With limited treatment for the picornaviruses, a detailed understanding of the structure is important to finding potential therapeutic targets. Two complimentary replication platforms, the cloverleaf of either the viral genome (5’CL) or the template strand (3’CL), could prove to be ideal targets. Previous work has shown distinct structural features of the 5’CL, but little is known about the 3’CL structure. We aim to determine the structure of the two stemloops (SLB and SLD) and the full 3’CL for Coxsackievirus B3 using Nuclear Magnetic Resonance (NMR) and Small-Angle X-Ray Scattering (SAXS). However, prior to analysis the samples must first be produced via T7 in-vitro transcription. This process, and its optimization, will be explored in this presentation.

Presenting Author Name/s

Meagan Werner

Faculty Advisor/Mentor

Steven Pascal

Faculty Advisor/Mentor Department

Chemistry and Biochemistry

College Affiliation

College of Sciences

Presentation Type

Poster

Disciplines

Biochemistry

Session Title

Poster Session

Location

Learning Commons Lobby @ Perry Library

Start Date

3-30-2024 8:30 AM

End Date

3-30-2024 10:00 AM

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Mar 30th, 8:30 AM Mar 30th, 10:00 AM

Optimizing In-Vitro Transcription of a Coxsackievirus RNA Replication Element

Learning Commons Lobby @ Perry Library

Picornaviruses are small RNA viruses responsible for illnesses such as hand-foot-mouth disease, poliomyelitis, and the common cold. With limited treatment for the picornaviruses, a detailed understanding of the structure is important to finding potential therapeutic targets. Two complimentary replication platforms, the cloverleaf of either the viral genome (5’CL) or the template strand (3’CL), could prove to be ideal targets. Previous work has shown distinct structural features of the 5’CL, but little is known about the 3’CL structure. We aim to determine the structure of the two stemloops (SLB and SLD) and the full 3’CL for Coxsackievirus B3 using Nuclear Magnetic Resonance (NMR) and Small-Angle X-Ray Scattering (SAXS). However, prior to analysis the samples must first be produced via T7 in-vitro transcription. This process, and its optimization, will be explored in this presentation.