Date of Award

Spring 1988

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

Patricia A. Pleban

Committee Member

W. A. Heaton

Committee Member

Kenneth G. Brown

Call Number for Print

Special Collections LD4331.C45H34

Abstract

Chromium-52 has recently been suggested for use as a new agent to label red cells for the in vivo quantitation of red cell volume (1). In this paper, the development and validation of a routine 52cr labeling procedure is described.

The accuracy, precision, and detection limits of chromium analysis by Zeeman effect atomic absorption spectroscopy was evaluated in the concentration range of 1 - 10 ug Cr/L.

Red cell chromium uptake was evaluated as a function of time, temperature, and concentration. Red cells labeled with a 2.5 mg/L chromium solution for 30 minutes at room temperature exhibited optimal label uptakes for the parameters studied. From these results, a minimal chromium dose was identified to achieve accurate in vivo quantitation.

Glutathione reductase and peroxidase enzyme activities, and cellular osmotic fragility were assayed as a function of chromium concentration. Glutathione peroxidase activity and osmotic fragility of cells collected in ACD solution were not significantly affected to chromium labeling concentrations of 25 mg/L. Glutathione reductase activities were reduced by approximately 14 % at 2.5 mg Cr/L, which is not significant clinically.

The accuracy of the developed 52cr labeling procedure was evaluated in vivo in eight subjects by simultaneous determination of red cell volume using 1251-Albumin, 51cr, 52cr, and the Dubois-Hurley method of red cell volume estimation (2). The results provide conclusive evidence that chromium-52 can be used for the accurate, quantitative determination of red cell volume by the developed procedure.

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DOI

10.25777/5f8x-jn09

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