Date of Award

Fall 1988

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry & Biochemistry

Program/Concentration

Chemistry

Committee Director

James H. Yuan

Committee Member

Mark S. Elliot

Committee Member

Roy L. Williams

Call Number for Print

Special Collections LD4331.C45K86

Abstract

A large scale preparation of human chorionic gonadotropin (HCG) from the urine of pregnant women was accomplished by using kaolin adsorption, NH4OH extraction and DEAE-cellulose ion exchange. A recovery about 81% of HCG and a specific activity in the 2,000-3,000 IU/mg range was achieved using this purification method.

Two HCG activity fractions, HCG I & HCG II, were isolated from DEAE-cellulose chromatography by a stepwise sodium chloride gradient elution. HCG I was eluted at 0.01-0.02 M NaCl while HCG II was eluted at 0.12-0.02 M NaCl.

The differences between HCG I and HCG II have been studied. The results show that HCG I has a molecular weight which is smaller than that of the native HCG and probably represents an intermediate in HCG metabolism which is excreted in the urine. The molecular weight of HCG II is estimated to be 63,000 ± 6,000 by gel filtration on a Sephadex G-200 column and 36,000 ± 3,000 by sucrose density gradient centrifugation. The estimate of molecular weight by gel filtration is inaccurate due to size changes which occur in glycoproteins, such as HCG, during the separation.

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In Copyright. URI: http://rightsstatements.org/vocab/InC/1.0/ This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

DOI

10.25777/8xh1-e103

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