Protoplasts were isolated from five species of Gossypium. Protoplast yield and viability were affected by incubation conditions, osmolarity, purification procedures, and cell source. Using an optimized procedure, highly viable protoplasts were isolated from cell suspensions, callus cultures, and leaf tissue of G. hirsutum, G. arboreum, G. klotzschianum, G. harknessii, and G. herbaceum. Protoplasts of G. harknessii were enucleated and successfully fused with protoplasts of G. hirsutum.
Renfroe, Michael H.; Hartwig, Ronald C.; and Smith, Roberta H.
"Isolation and Fusion of Cotton Protoplasts,"
Virginia Journal of Science: Vol. 52
, Article 5.
Available at: http://digitalcommons.odu.edu/vjs/vol52/iss1/5